Victory in Spain will ensure that Connacht finish as number 1 seeds for the Amlin Challenge Cup quarter-finals, having secured their qualification from Pool 2 last weekend.
Speaking ahead of the trip to Spain, Connacht coach Michael Bradley said: "It's important we win the match and go on then and get the bonus point, but we are travelling strong.
"I would be very surprised if that happened - it's too big an opportunity and the quality of players currently here is better than it was back them."
CONNACHT (v Olympus Rugby Madrid): Troy Nathan; Liam Bibo, Niva Ta'auso, Keith Matthews, Fionn Carr; Ian Keatley, Frank Murphy; Ronan Loughney, Sean Cronin, Robbie Morris, Michael Swift, Michael McCarthy, John Muldoon (capt), Johnny O'Connor, George Naoupu.
Replacements: Adrian Flavin, Brett Wilkinson, Jamie Hagan, Bernie Upton, Mike McComish, Conor O'Loughlin, Miah Nikora, Aidan Wynne.