Gavin Duffy moves from the senior squad to the Wolfhounds and will start at full-back, while Fergus McFadden has been called into the senior squad as cover with Ian Whitten replacing him for the trip to Scotland.
Ian Nagle will start for the Wolfhounds on Friday as both Donnacha Ryan and Dan Tuohy, who were named in the original squad, have been ruled out because of injury.
Munster lock Nagle will be partnered in the second row by Devin Toner, who recently extended his contract with Leinster.
Meanwhile, after the Scotland trip, the Ireland Wolfhounds will be back on duty at Ravenhill on Friday, February 4 when they entertain England Saxons. Ticket are available to purchase here.
O2 IRELAND WOLFHOUNDS Team & Replacements (v Scotland 'A', 'A' International Match, Netherdale, Friday, January 28, kick-off 7.30pm):
15. Gavin Duffy (Galwegians/Connacht)
14. Johne Murphy (Young Munster/Munster)
13. Eoin O'Malley (Old Belvedere/Leinster)
12. Nevin Spence (Ballynahinch/Ulster)
11. Denis Hurley (Cork Constitution/Munster)
10. Ian Humphreys (Ballymena/Ulster)
1. Brett Wilkinson (Galwegians/Connacht)
2. Damien Varley (Garryowen/Munster)
3. Tony Buckley (Shannon/Munster)
4. Ian Nagle (Cork Constitution/Munster)
5. Devin Toner (Lansdowne/Leinster)
6. Kevin McLaughlin (St. Mary's College/Leinster)
7. Willie Faloon (Ballynahinch/Ulster)
8. Chris Henry (Ballymena/Ulster) (capt)
16. Nigel Brady (Dungannon/Ulster)
17. Jamie Hagan (Corinthians/Connacht)
18. Andrew Browne (Galwegians/Connacht)
19. Dominic Ryan (Lansdowne/Leinster)
20. Isaac Boss (Terenure College/Leinster)
21. Niall O'Connor (Belfast Harlequins/Ulster)
22. Ian Whitten (Ballynahinch/Ulster)