Semi-final 1: Munster v Ulster
Semi-final 2: Cornish Pirates v Doncaster Knights
The semi-finals will take place over the weekend of April 23 to 25.
The winner of semi-final 2 will have home advantage in the final, which will take place on Sunday, May 16 at 6pm, live on Sky Sports HD.
British & Irish Cup tournament director Terry Burwell said: "We now have two mouth-watering semi-finals for players, coaches and supporters of these clubs and provinces to look forward to
"There will also be an Anglo-Irish final live on Sky Sports HD which will further showcase this exciting new cross-border competition."
-12 English Championship clubs (Bedford, Birmingham & Solihull, Bristol Rugby, Cornish Pirates, Coventry, Doncaster Knights, Exeter Chiefs, London Welsh, Moseley, Nottingham, Plymouth Albion and Rotherham Titans)
- Top 6 Welsh Principality Premiership clubs (Aberavon, Cardiff, Llanelli, Neath, Newport and Pontypridd)
- 3 Irish provinces (Munster Leinster and Ulster)
- 3 Scottish teams - top two finishers in Scottish Hydro Electric Premiership Division 1 (champions Ayr and runners-up Heriot's) and the Scottish Thistles, a team drawn from Edinburgh and Glasgow and members of Scottish Rugby's National Academy