Friday will see the westerners bidding to continue their proud record of having won every match on the opening day of the Magners League season since the competition began.
Connacht's new captain John Muldoon said: "Our pre-season has gone well but we now need to take that into competitive action.
"Everyone is dying for the Magners League to get underway and we're hopeful that we can make a good start, beginning with Friday's game.
However, the hosts will be missing a number of squad regulars for their first competitive game of the campaign.
Mel Deane (foot), Tim Donnelly (shoulder), Andy Dunne (hamstring), Johnny O'Connor (hand), Conor O'Loughlin (hip) and recent signing Mike Roberts (calf) are all nursing knocks, while David Gannon and Danny Riordan are also notable absentees.
CONNACHT Squad (v Ospreys): Backs (10) - Liam Bibo, Kieran Campbell, Fionn Carr, Gavin Duffy, John Hearty, Ian Keatley, Keith Matthews, Frank Murphy, Troy Nathan, Aidan Wynne.
Forwards (12) - Andrew Browne, Sean Cronin, Andrew Farley, Adrian Flavin, Ronan Loughney, Michael McCarthy, Robbie Morris, John Muldoon (capt), Ray Ofisa, Colm Rigney, Michael Swift, Brett Wilkinson.